Copper oxide nanoparticles recruit macrophages and modulate nitric oxide, proinflammatory cytokines and PGE2 production through arginase activation.
Nanomedicine (Lond)
; 11(10): 1237-51, 2016 05.
Article
en En
| MEDLINE
| ID: mdl-27079258
ABSTRACT
AIM:
In the present study, we examine the effects of copper oxide nanoparticles (CuNP) on macrophage immune response and the signaling pathways involved. MATERIALS &METHODS:
A peritonitis model was used to determine in vivo immune cells recruitment, while primary macrophages were used as an in vitro model for the cellular and molecular analysis.RESULTS:
In vivo, CuNP induce significant macrophages recruitment to the site of injection. In vitro, in LPS-stimulated primary macrophages, the co-treatment with CuNP inhibited the production of NO in a dose-dependent manner. The mechanism underlying NO and proinflammatory cytokines inhibition was associated with an increased arginase activity. Macrophage stimulation with CuNP did not provoke any cytokine secretion; however, arginase inhibition promoted TNFα and MIP-1ß production. In addition, CuNP induced the expression of COX-2 and the production of PGE2 through arginase activation.CONCLUSION:
Our results demonstrate that CuNP activate arginase and suppress macrophage innate immune response.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Arginasa
/
Dinoprostona
/
Citocinas
/
Cobre
/
Nanopartículas
/
Macrófagos
/
Óxido Nítrico
Límite:
Animals
Idioma:
En
Revista:
Nanomedicine (Lond)
Año:
2016
Tipo del documento:
Article
País de afiliación:
Chile