IL-32α has differential effects on proliferation and apoptosis of human melanoma cell lines.
J Surg Oncol
; 113(4): 364-9, 2016 Mar.
Article
en En
| MEDLINE
| ID: mdl-27100023
ABSTRACT
BACKGROUND:
Interleukin-32 (IL-32) is a recently recognized intracellular, proinflammatory cytokine which may play a role in cancer metastasis and patient survival. The role of IL-32 in cancer, especially its direct effect on cancer cells, is not well understood. MATERIAL ANDMETHODS:
Clonogenic assay, PCNA staining, Quick Cell Proliferation assay, TUNEL staining, and caspase-3 activity assay were used to investigate the in vitro role for IL-32α in human melanoma growth. We further investigated the possible molecular mechanisms using RT-PCR and immunohistochemical staining.RESULTS:
Exogenous administration of IL-32α inhibited proliferation of the HTB-72 human melanoma cell line, but had little effect on other melanoma cell lines. Inhibition of proliferation in HTB-72 correlated with increased expression of p21 and p53. IL-32α administration also increased apoptosis in HTB-72. This finding correlated with increased expression of TRAILR1.CONCLUSIONS:
The data presented suggest a direct effect of IL-32α on the growth of human melanoma and give some insight into the mechanisms which may in part govern this effect. J. Surg. Oncol. 2016;113364-369. © 2016 Wiley Periodicals, Inc.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Interleucinas
/
Melanoma
Límite:
Humans
Idioma:
En
Revista:
J Surg Oncol
Año:
2016
Tipo del documento:
Article