Superresolution Microscopy of the Nuclear Envelope and Associated Proteins.
Methods Mol Biol
; 1411: 83-97, 2016.
Article
en En
| MEDLINE
| ID: mdl-27147035
ABSTRACT
Superresolution microscopy is undoubtedly one of the most exciting technologies since the invention of the optical microscope. Capable of nanometer-scale resolution to surpass the diffraction limit and coupled with the versatile labeling techniques available, it is revolutionizing the study of cell biology. Our understanding of the nucleus, the genetic and architectural center of the cell, has gained great advancements through the application of various superresolution microscopy techniques. This chapter describes detailed procedures of multichannel superresolution imaging of the mammalian nucleus, using structured illumination microscopy and single-molecule localization microscopy.
Palabras clave
Linker of the nucleoskeleton and cytoskeleton (LINC) complex; Nuclear envelope (NE); Nuclear lamins; Nuclear pore complex (NPC); Photoactivated localization microscopy (PALM); Single-molecule localization microscopy (SMLM); Stochastic optical reconstruction microscopy (STORM); Structured illumination microscopy (SIM); Superresolution microscopy (SRM); Synaptonemal complex
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas de la Membrana
/
Microscopía
/
Membrana Nuclear
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2016
Tipo del documento:
Article
País de afiliación:
Singapur