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A validated method for rapid determination of dibenzo-p-dioxins/furans (PCDD/Fs), polybrominated diphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs) in human milk: focus on utility of tandem solid phase extraction (SPE) cleanup.
Lin, Yuanjie; Feng, Chao; Xu, Qian; Lu, Dasheng; Qiu, Xinlei; Jin, Yu'e; Wang, Guoquan; Wang, Dongli; She, Jianwen; Zhou, Zhijun.
Afiliación
  • Lin Y; Shanghai Municipal Center for Disease Control and Prevention, 1380 Zhongshan West Road, Shanghai, 200336, China.
  • Feng C; Shanghai Municipal Center for Disease Control and Prevention, 1380 Zhongshan West Road, Shanghai, 200336, China.
  • Xu Q; Shanghai Municipal Center for Disease Control and Prevention, 1380 Zhongshan West Road, Shanghai, 200336, China.
  • Lu D; Shanghai Municipal Center for Disease Control and Prevention, 1380 Zhongshan West Road, Shanghai, 200336, China. ludasheng@scdc.sh.cn.
  • Qiu X; School of Public Health/MOE Key Lab for Public Health/Collaborative Innovation Center of Social Risks Governance in Health, Fudan University, 130 Dong'an Road, Shanghai, 200032, China. ludasheng@scdc.sh.cn.
  • Jin Y; Shanghai Municipal Center for Disease Control and Prevention, 1380 Zhongshan West Road, Shanghai, 200336, China.
  • Wang G; Shanghai Municipal Center for Disease Control and Prevention, 1380 Zhongshan West Road, Shanghai, 200336, China.
  • Wang D; Shanghai Municipal Center for Disease Control and Prevention, 1380 Zhongshan West Road, Shanghai, 200336, China. wangguoquan@scdc.sh.cn.
  • She J; California Department of Public Health, 850 Marina Bay Parkway, Richmond, CA, 94804, USA.
  • Zhou Z; California Department of Public Health, 850 Marina Bay Parkway, Richmond, CA, 94804, USA.
Anal Bioanal Chem ; 408(18): 4897-906, 2016 Jul.
Article en En | MEDLINE | ID: mdl-27178547
ABSTRACT
An improved method based on tandem solid phase extraction (SPE) cleanup and gas chromatography-high resolution mass spectrometry (GC-HRMS) has been validated for a rapid determination of dibenzo-p-dioxins/furans (PCDD/Fs), dioxin-like polychlorinated biphenyls (PCBs), marker polychlorinated biphenyls (M-PCBs), and polybrominated diphenyl ethers (PBDEs) using a large volume (50 mL) of human milk. This method was well validated for the measurement of these analytes in human milk from the general population with low limits of detection (LODs, 0.004-0.12 ng/g lipid), satisfactory accuracy (75-120 % of recoveries), and precision [less than 10 % of relative standard deviations (RSDs)]. To comprehensively evaluate the performance of this method, a good, presently validated and routinely used method based on an automated sample clean-up system (ASCS, based on the commercial acid multilayer silica, basic alumina, and carbon columns) was used in parallel for comparison. Compared with the ASCS method, this method presented comparable specificity. Additionally, this method, in contrast to ASCS method, highly reduced consumption of solvents (40 mL versus 500 mL), which results in much lower background in the procedural blank, reduced time, and enhanced sample pretreatment throughput. This method was also applied in a pilot study to measure a batch of human milk samples with satisfactory results. Graphical Abstract Characteristics of the application of tandem SPE cleanup for determination of PCDD/Fs, DL-PCBs,M-PCBs and PBDEs in human milk.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Contaminación de Alimentos / Leche / Contaminantes Ambientales / Espectrometría de Masas en Tándem / Extracción en Fase Sólida / Análisis de los Alimentos / Hidrocarburos Aromáticos Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Animals / Humans Idioma: En Revista: Anal Bioanal Chem Año: 2016 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Contaminación de Alimentos / Leche / Contaminantes Ambientales / Espectrometría de Masas en Tándem / Extracción en Fase Sólida / Análisis de los Alimentos / Hidrocarburos Aromáticos Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Animals / Humans Idioma: En Revista: Anal Bioanal Chem Año: 2016 Tipo del documento: Article País de afiliación: China