Determination of blood cell subtype concentrations from frozen whole blood samples using TruCount beads.
Cytometry B Clin Cytom
; 94(4): 660-666, 2018 07.
Article
en En
| MEDLINE
| ID: mdl-27342079
ABSTRACT
BACKGROUND:
In many studies it would be advantageous if blood samples could be collected and analyzed using flow cytometry at a later stage. Ideally, sample collection should involve little hands-on time, allow for long-term storage, and minimally influence the samples.METHODS:
Here we establish a flow cytometry antibody panel that can be used to determine granulocytes, monocytes, and lymphocyte subset concentrations in fresh and frozen whole blood using TruCount technology.RESULTS:
The panel can be used on fresh whole-blood samples as well as whole-blood samples that have been frozen after mixing with 10% DMSO. Concentrations in frozen and fresh sample is highly correlated both when frozen within 4 h and the day after collection (r ≥ 0.98), and the estimated concentration in frozen samples was between 91 and 94% of that in fresh samples for all cell types.CONCLUSION:
Using this method whole-blood samples can be frozen using a simple preparation method, and stored long-term before accurate determination of cell concentration. This allows for standardized analysis of the samples at a reference laboratory in multi-center studies. © 2016 International Clinical Cytometry Society.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Criopreservación
/
Inmunofenotipificación
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Citometría de Flujo
/
Recuento de Leucocitos
Tipo de estudio:
Clinical_trials
Límite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Cytometry B Clin Cytom
Año:
2018
Tipo del documento:
Article
País de afiliación:
Suecia