Identifying Kinase Substrates via a Heavy ATP Kinase Assay and Quantitative Mass Spectrometry.
Sci Rep
; 6: 28107, 2016 06 27.
Article
en En
| MEDLINE
| ID: mdl-27346722
ABSTRACT
Mass spectrometry-based in vitro kinase screens play an essential role in the discovery of kinase substrates, however, many suffer from biological and technical noise or necessitate genetically-altered enzyme-cofactor systems. We describe a method that combines stable γ-[(18)O2]-ATP with classical in vitro kinase assays within a contemporary quantitative proteomic workflow. Our approach improved detection of known substrates of the non-receptor tyrosine kinase ABL1; and identified potential, new in vitro substrates.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Espectrometría de Masas
/
Adenosina Trifosfato
/
Proteínas del Citoesqueleto
/
Proteínas Adaptadoras Transductoras de Señales
Límite:
Humans
Idioma:
En
Revista:
Sci Rep
Año:
2016
Tipo del documento:
Article
País de afiliación:
Austria