Age-associated hydroxymethylation in human bone-marrow mesenchymal stem cells.
J Transl Med
; 14(1): 207, 2016 07 08.
Article
en En
| MEDLINE
| ID: mdl-27393146
ABSTRACT
BACKGROUND:
Age-associated changes in genomic DNA methylation have been primarily attributed to 5-methylcytosine (5mC). However, the recent discovery of 5-hydroxymethylcytosine (5hmC) suggests that this epigenetic mark might also play a role in the process.METHODS:
Here, we analyzed the genome-wide profile of 5hmc in mesenchymal stem cells (MSCs) obtained from bone-marrow donors, aged 2-89 years.RESULTS:
We identified 10,685 frequently hydroxymethylated CpG sites in MSCs that were, as in other cell types, significantly associated with low density CpG regions, introns, the histone posttranslational modification H3k4me1 and enhancers. Study of the age-associated changes to 5hmC identified 785 hyper- and 846 hypo-hydroxymethylated CpG sites in the MSCs obtained from older individuals.CONCLUSIONS:
DNA hyper-hydroxymethylation in the advanced-age group was associated with loss of 5mC, which suggests that, at specific CpG sites, this epigenetic modification might play a role in DNA methylation changes during lifetime. Since bone-marrow MSCs have many clinical applications, and the fact that the epigenomic alterations in this cell type associated with aging identified in this study could have associated functional effects, the age of donors should be taken into account in clinical settings.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Envejecimiento
/
Células de la Médula Ósea
/
Metilación de ADN
/
5-Metilcitosina
/
Células Madre Mesenquimatosas
Tipo de estudio:
Risk_factors_studies
Límite:
Adolescent
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Adult
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Aged
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Aged80
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Child
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Child, preschool
/
Humans
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Middle aged
Idioma:
En
Revista:
J Transl Med
Año:
2016
Tipo del documento:
Article
País de afiliación:
España