Differential long noncoding RNA and mRNA expression in differentiated human glioblastoma stem cells.
Mol Med Rep
; 14(3): 2067-76, 2016 Sep.
Article
en En
| MEDLINE
| ID: mdl-27432080
ABSTRACT
Differentiation of glioblastoma stem cells (GSCs) may lead to inhibition of their selfrenewing ability and tumorigenic potential, as well as increasing their sensitivity to treatment. The critical role of long noncoding RNAs (lncRNAs) in numerous biological processes has been revealed. However, the involvement of lncRNAs in GSC differentiation remains to be elucidated. In the present study, GSCs were isolated from patient samples and differentiation was induced. Using a highthroughput microarray, the present study identified a profile of 1,545 lncRNAs and 2,729 mRNAs that differed between GSCs and their nondifferentiated counterparts. To ascertain the association between the altered lncRNAs and mRNAs, a coexpression network was constructed in which 1,087 lncRNAs and 1,928 mRNAs altered upon GSC differentiation formed a total of 19,642 lncRNAmRNA pairs. Based on the coexpression network, the lncRNA functions were additionally predicted by a cis or trans targeting program. Furthermore, three pairs of lncRNAs and their nearby target mRNAs were selected [ENSG00000261924.1regulatory associated protein of MTOR complex 1, ENSG00000235427.1caveolin 1 and Tax1 binding protein 3 (TAX1BP3)purinergic receptor P2X 5 (P2RX5)TAX1BP3] and their expression levels were validated by reverse transcriptionquantitative polymerase chain reaction. The altered lncRNAs were also regulated by various pluripotency transcription factors (POU domain, class 3, transcription factor, sex determining region Ybox 2, spaltlike transcription factor 2 and oligodendrocyte lineage transcription factor 2). In conclusion, the results of the present study revealed that lncRNAs may function in GSC differentiation by regulating their target mRNAs, and a set of lncRNAs were identified as candidates for further study concerning the future treatment of GSCs.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Células Madre Neoplásicas
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ARN Mensajero
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Regulación Neoplásica de la Expresión Génica
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Glioblastoma
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ARN Largo no Codificante
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Mol Med Rep
Año:
2016
Tipo del documento:
Article