Aneuploid embryonic stem cells exhibit impaired differentiation and increased neoplastic potential.
EMBO J
; 35(21): 2285-2300, 2016 11 02.
Article
en En
| MEDLINE
| ID: mdl-27558554
ABSTRACT
Aneuploidy leads to severe developmental defects in mammals and is also a hallmark of cancer. However, whether aneuploidy is a driving cause or a consequence of tumor formation remains controversial. Paradoxically, existing studies based on aneuploid yeast and mouse fibroblasts have shown that aneuploidy is usually detrimental to cellular fitness. Here, we examined the effects of aneuploidy on mouse embryonic stem (ES) cells by generating a series of cell lines that each carries an extra copy of single chromosomes, including trisomy 6, 8, 11, 12, or 15. Most of these aneuploid cell lines had rapid proliferation rates and enhanced colony formation efficiencies. They were less dependent on growth factors for self-renewal and showed a reduced capacity to differentiate in vitro Moreover, trisomic stem cells formed teratomas more efficiently, from which undifferentiated cells can be recovered. Further investigations demonstrated that co-culture of wild-type and aneuploid ES cells or supplementation with extracellular BMP4 rescues the differentiation defects of aneuploid ES cells.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Teratoma
/
Trisomía
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Diferenciación Celular
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Transformación Celular Neoplásica
/
Células Madre Embrionarias
Límite:
Animals
Idioma:
En
Revista:
EMBO J
Año:
2016
Tipo del documento:
Article
País de afiliación:
China