Development of Next Generation Synthetic Biology Tools for Use in Streptomyces venezuelae.
ACS Synth Biol
; 6(1): 159-166, 2017 01 20.
Article
en En
| MEDLINE
| ID: mdl-27605473
ABSTRACT
Streptomyces have a rich history as producers of important natural products and this genus of bacteria has recently garnered attention for its potential applications in the broader context of synthetic biology. However, the dearth of genetic tools available to control and monitor protein production precludes rapid and predictable metabolic engineering that is possible in hosts such as Escherichia coli or Saccharomyces cerevisiae. In an effort to improve genetic tools for Streptomyces venezuelae, we developed a suite of standardized, orthogonal integration vectors and an improved method to monitor protein production in this host. These tools were applied to characterize heterologous promoters and various attB chromosomal integration sites. A final study leveraged the characterized toolset to demonstrate its use in producing the biofuel precursor bisabolene using a chromosomally integrated expression system. These tools advance S. venezuelae to be a practical host for future metabolic engineering efforts.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Streptomyces
Tipo de estudio:
Prognostic_studies
País/Región como asunto:
America do sul
/
Venezuela
Idioma:
En
Revista:
ACS Synth Biol
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos