The Rab3A-22A Chimera Prevents Sperm Exocytosis by Stabilizing Open Fusion Pores.
J Biol Chem
; 291(44): 23101-23111, 2016 10 28.
Article
en En
| MEDLINE
| ID: mdl-27613869
ABSTRACT
At the final stage of exocytotis, a fusion pore opens between the plasma and a secretory vesicle membranes; typically, when the pore dilates the vesicle releases its cargo. Sperm contain a large dense-core secretory granule (the acrosome) whose contents are secreted by regulated exocytosis at fertilization. Minutes after the arrival of the triggering signal, the acrosomal and plasma membranes dock at multiple sites and fusion pores open at the contact points. It is believed that immediately afterward, fusion pores dilate spontaneously. Rab3A is an essential component of human sperm exocytotic machinery. Yet, recombinant, persistently active Rab3A halts calcium-triggered secretion when introduced after docking into streptolysin O-permeabilized cells; so does a Rab3A-22A chimera. Here, we applied functional assays, electron and confocal microscopy to show that the secretion blockage is due to the stabilization of open fusion pores. Other novel findings are that sperm SNAREs engage in α-SNAP/NSF-sensitive complexes at a post-fusion stage. Complexes are disentangled by these chaperons to achieve vesiculation and acrosomal contents release. Thus, post-fusion regulation of the pores determines their expansion and the success of the acrosome reaction.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Espermatozoides
/
Proteínas de Unión al GTP rab
/
Proteína de Unión al GTP rab3A
/
Exocitosis
Límite:
Humans
/
Male
Idioma:
En
Revista:
J Biol Chem
Año:
2016
Tipo del documento:
Article
País de afiliación:
Argentina