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Transcriptional regulation analysis of FAM3A gene and its effect on adipocyte differentiation.
Kang, Tingting; Peng, Disha; Bu, Guowei; Gu, Hao; Zhang, Feng; Zhang, Ruirui; Zhou, Ying; Xiong, Yuanzhu; Lei, Minggang.
Afiliación
  • Kang T; Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of
  • Peng D; Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of
  • Bu G; Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of
  • Gu H; Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of
  • Zhang F; Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Ve
  • Zhang R; Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of
  • Zhou Y; Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of
  • Xiong Y; Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of
  • Lei M; Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of
Gene ; 595(1): 92-98, 2016 Dec 20.
Article en En | MEDLINE | ID: mdl-27688071
ABSTRACT
FAM3A (family with sequence similarity 3, member A) is regulated by PPARG and participates in the metabolism of lipid in liver. However, the transcriptional regulation analysis of FAM3A is very little and biological function of FAM3A still unclear. In this study, the core promoter region and transcription factor binding sites of FAM3A gene were identified and characterized using dual luciferase report experiments and electrophoretic mobility shift assays (EMSA). The promoter activity of FAM3A was dramatically decreased after the mutation of C/EBPß binding sites, suggesting that C/EBPß is a transcriptional activator of FAM3A. Overexpression of FAM3A significantly inhibited the efficiency of preadipocytes to differentiate into adipocytes as indicated by Western Blot and Oil Red O staining assay. These results suggest that C/EBPß plays an important role in regulating FAM3A promoter activity and FAM3A inhibits adipocyte differentiation.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Transcripción Genética / Diferenciación Celular / Citocinas / Adipocitos / Elementos de Respuesta Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Gene Año: 2016 Tipo del documento: Article
Texto completo
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Transcripción Genética / Diferenciación Celular / Citocinas / Adipocitos / Elementos de Respuesta Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Gene Año: 2016 Tipo del documento: Article