Development and evaluation of a rapid recombinase polymerase amplification assay for detection of coxsackievirus A6.
Arch Virol
; 162(1): 287-290, 2017 Jan.
Article
en En
| MEDLINE
| ID: mdl-27722991
ABSTRACT
Coxsackievirus A6 (CV-A6) is an important pathogen causing hand, foot and mouth disease (HFMD). The aim of this study was to develop and evaluate a rapid real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of CV-A6. The sensitivity of this assay was 202 copies/reaction, with 100 % specificity. Furthermore, this assay yielded consistent results comparable with a commercial qRT-PCR diagnostic kit. This assay is therefore potentially useful for surveillance of CV-A6 infections and outbreak control.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Enterovirus
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Técnicas de Amplificación de Ácido Nucleico
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Reacción en Cadena en Tiempo Real de la Polimerasa
/
Enfermedad de Boca, Mano y Pie
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Child, preschool
/
Humans
/
Infant
/
Newborn
Idioma:
En
Revista:
Arch Virol
Año:
2017
Tipo del documento:
Article
País de afiliación:
China