Characterizing the mitochondrial DNA polymerase gamma interactome by BioID identifies Ruvbl2 localizes to the mitochondria.
Mitochondrion
; 32: 31-35, 2017 Jan.
Article
en En
| MEDLINE
| ID: mdl-27845271
ABSTRACT
Human mitochondrial DNA (mtDNA) is replicated by the mitochondrial DNA polymerase gamma (POLG). Using proximity dependent biotin labelling (BioID), we characterized the POLG interactome and identified new interaction partners involved in mtDNA maintenance, transcription, translation and protein quality control. We also identified interaction with the nuclear AAA+ ATPase Ruvbl2, suggesting mitochondrial localization for this protein. Ruvbl2 was detected in mitochondria-enriched fractions in leukemic cells. Additionally, transgenic overexpression of Ruvbl2 from an alternative translation initiation site resulted in mitochondrial co-localization. Overall, POLG interactome mapping identifies novel proteins which support mitochondrial biogenesis and a potential novel mitochondrial isoform of Ruvbl2.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Portadoras
/
ADN Helicasas
/
Mapeo de Interacción de Proteínas
/
ADN Polimerasa Dirigida por ADN
/
Mitocondrias
Límite:
Humans
Idioma:
En
Revista:
Mitochondrion
Año:
2017
Tipo del documento:
Article
País de afiliación:
Canadá