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Characterizing the mitochondrial DNA polymerase gamma interactome by BioID identifies Ruvbl2 localizes to the mitochondria.
Liyanage, Sanduni U; Coyaud, Etienne; Laurent, Estelle M N; Hurren, Rose; Maclean, Neil; Wood, Stuart R; Kazak, Lawrence; Shamas-Din, Aisha; Holt, Ian; Raught, Brian; Schimmer, Aaron.
Afiliación
  • Liyanage SU; Princess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada; Department of Medical Biophysics, University of Toronto, ON, Canada.
  • Coyaud E; Princess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada.
  • Laurent EM; Princess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada.
  • Hurren R; Princess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada.
  • Maclean N; Princess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada.
  • Wood SR; Medical Research Council, Mitochondrial Biology Unit, Cambridge, UK.
  • Kazak L; Medical Research Council, Mitochondrial Biology Unit, Cambridge, UK.
  • Shamas-Din A; Princess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada.
  • Holt I; Medical Research Council, Mitochondrial Biology Unit, Cambridge, UK.
  • Raught B; Princess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada.
  • Schimmer A; Princess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada; Department of Medical Biophysics, University of Toronto, ON, Canada. Electronic address: aaron.schimmer@utoronto.ca.
Mitochondrion ; 32: 31-35, 2017 Jan.
Article en En | MEDLINE | ID: mdl-27845271
ABSTRACT
Human mitochondrial DNA (mtDNA) is replicated by the mitochondrial DNA polymerase gamma (POLG). Using proximity dependent biotin labelling (BioID), we characterized the POLG interactome and identified new interaction partners involved in mtDNA maintenance, transcription, translation and protein quality control. We also identified interaction with the nuclear AAA+ ATPase Ruvbl2, suggesting mitochondrial localization for this protein. Ruvbl2 was detected in mitochondria-enriched fractions in leukemic cells. Additionally, transgenic overexpression of Ruvbl2 from an alternative translation initiation site resulted in mitochondrial co-localization. Overall, POLG interactome mapping identifies novel proteins which support mitochondrial biogenesis and a potential novel mitochondrial isoform of Ruvbl2.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Portadoras / ADN Helicasas / Mapeo de Interacción de Proteínas / ADN Polimerasa Dirigida por ADN / Mitocondrias Límite: Humans Idioma: En Revista: Mitochondrion Año: 2017 Tipo del documento: Article País de afiliación: Canadá
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Portadoras / ADN Helicasas / Mapeo de Interacción de Proteínas / ADN Polimerasa Dirigida por ADN / Mitocondrias Límite: Humans Idioma: En Revista: Mitochondrion Año: 2017 Tipo del documento: Article País de afiliación: Canadá