Genomewide association study of peanut allergy reproduces association with amino acid polymorphisms in HLA-DRB1.
Clin Exp Allergy
; 47(2): 217-223, 2017 Feb.
Article
en En
| MEDLINE
| ID: mdl-27883235
ABSTRACT
BACKGROUND:
Genetic variants for IgE-mediated peanut allergy are yet to be fully characterized and to date only one genomewide association study (GWAS) has been published.OBJECTIVE:
To identify genetic variants associated with challenge-proven peanut allergy.METHODS:
We carried out a GWAS comparing 73 infants with challenge-proven IgE-mediated peanut allergy against 148 non-allergic infants (all ~ 1 year old). We tested a total of 3.8 million single nucleotide polymorphisms, as well as imputed HLA alleles and amino acids. Replication was assessed by de novo genotyping in a panel of additional 117 cases and 380 controls, and in silico testing in two independent GWAS cohorts.RESULTS:
We identified 21 independent associations at P ≤ 5 × 10-5 but were unable to replicate these. The most significant HLA association was the previously reported amino acid variant located at position 71, within the peptide-binding groove of HLA-DRB1 (P = 2 × 10-4 ). Our study therefore reproduced previous findings for the association between peanut allergy and HLA-DRB1 in this Australian population. CONCLUSIONS AND CLINICAL RELEVANCE Genetic determinants for challenge-proven peanut allergy include alleles at the HLA-DRB1 locus.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Polimorfismo Genético
/
Sustitución de Aminoácidos
/
Predisposición Genética a la Enfermedad
/
Hipersensibilidad al Cacahuete
/
Estudio de Asociación del Genoma Completo
/
Cadenas HLA-DRB1
Tipo de estudio:
Etiology_studies
/
Prognostic_studies
/
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Clin Exp Allergy
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Australia