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The rice OsNAC6 transcription factor orchestrates multiple molecular mechanisms involving root structural adaptions and nicotianamine biosynthesis for drought tolerance.
Lee, Dong-Keun; Chung, Pil Joong; Jeong, Jin Seo; Jang, Geupil; Bang, Seung Woon; Jung, Harin; Kim, Youn Shic; Ha, Sun-Hwa; Choi, Yang Do; Kim, Ju-Kon.
Afiliación
  • Lee DK; Graduate School of International Agricultural Technology and Crop Biotechnology Institute/GreenBio Science and Technology, Seoul National University, Pyeongchang, Korea.
  • Chung PJ; Graduate School of International Agricultural Technology and Crop Biotechnology Institute/GreenBio Science and Technology, Seoul National University, Pyeongchang, Korea.
  • Jeong JS; Graduate School of International Agricultural Technology and Crop Biotechnology Institute/GreenBio Science and Technology, Seoul National University, Pyeongchang, Korea.
  • Jang G; Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
  • Bang SW; Graduate School of International Agricultural Technology and Crop Biotechnology Institute/GreenBio Science and Technology, Seoul National University, Pyeongchang, Korea.
  • Jung H; Graduate School of International Agricultural Technology and Crop Biotechnology Institute/GreenBio Science and Technology, Seoul National University, Pyeongchang, Korea.
  • Kim YS; Graduate School of International Agricultural Technology and Crop Biotechnology Institute/GreenBio Science and Technology, Seoul National University, Pyeongchang, Korea.
  • Ha SH; Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, Korea.
  • Choi YD; Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
  • Kim JK; Graduate School of International Agricultural Technology and Crop Biotechnology Institute/GreenBio Science and Technology, Seoul National University, Pyeongchang, Korea.
Plant Biotechnol J ; 15(6): 754-764, 2017 Jun.
Article en En | MEDLINE | ID: mdl-27892643
ABSTRACT
Drought has a serious impact on agriculture worldwide. A plant's ability to adapt to rhizosphere drought stress requires reprogramming of root growth and development. Although physiological studies have documented the root adaption for tolerance to the drought stress, underlying molecular mechanisms is still incomplete, which is essential for crop engineering. Here, we identified OsNAC6-mediated root structural adaptations, including increased root number and root diameter, which enhanced drought tolerance. Multiyear drought field tests demonstrated that the grain yield of OsNAC6 root-specific overexpressing transgenic rice lines was less affected by drought stress than were nontransgenic controls. Genome-wide analyses of loss- and gain-of-function mutants revealed that OsNAC6 up-regulates the expression of direct target genes involved in membrane modification, nicotianamine (NA) biosynthesis, glutathione relocation, 3'-phophoadenosine 5'-phosphosulphate accumulation and glycosylation, which represent multiple drought tolerance pathways. Moreover, overexpression of NICOTIANAMINE SYNTHASE genes, direct targets of OsNAC6, promoted the accumulation of the metal chelator NA and, consequently, drought tolerance. Collectively, OsNAC6 orchestrates novel molecular drought tolerance mechanisms and has potential for the biotechnological development of high-yielding crops under water-limiting conditions.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas de Plantas / Oryza / Factores de Transcripción / Raíces de Plantas Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Biotechnol J Asunto de la revista: BIOTECNOLOGIA / BOTANICA Año: 2017 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas de Plantas / Oryza / Factores de Transcripción / Raíces de Plantas Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Biotechnol J Asunto de la revista: BIOTECNOLOGIA / BOTANICA Año: 2017 Tipo del documento: Article