Hemoglobin induction by Ara-C in human erythroleukemic cells (K562) is cell-cycle dependent.
Leuk Res
; 13(8): 683-8, 1989.
Article
en En
| MEDLINE
| ID: mdl-2796376
ABSTRACT
This study was undertaken to answer the question of whether chemical induction of hemoglobin in the human erythroleukemic line K562 is cell-cycle dependent. Cells were synchronized with respect to the cell cycle by manipulation of culture conditions. S phase was detected microscopically by immunofluorescent staining. Hemin and arabinofuranosylcytosine (Ara-C) were used to induce hemoglobin synthesis in K562 cells. The action of hemin causes induction without specificity in regard to cell cycle phase, while Ara-C is dependent upon cell cycle phase, with cells within late G1 and early S phases being sensitive to the effect of the agent. These studies also confirm the results that the action of hemin is reversible but the induction caused by Ara-C is irreversible. The synergistic effects of these two inducing agents resulted in a sharper rise in the percentage of induced cells.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Hemoglobinas
/
Leucemia Eritroblástica Aguda
/
Ciclo Celular
/
Citarabina
Límite:
Humans
Idioma:
En
Revista:
Leuk Res
Año:
1989
Tipo del documento:
Article