Tetramethyl Pyrazine Protects Hippocampal Neurons Against Anoxia/Reoxygenation Injury Through Inhibiting Apoptosis Mediated by JNK/MARK Signal Pathway.
Med Sci Monit
; 22: 5082-5090, 2016 Dec 23.
Article
en En
| MEDLINE
| ID: mdl-28009855
ABSTRACT
BACKGROUND Tetramethyl pyrazine (TMP) is a typical biologically active alkaloid isolated from the Chinese herb Ligusticum walliichi. It has been reported that TMP shows neuroprotective and stroke injury reductive properties in cerebral ischemia/reperfusion (I/R) animal models. In the present study we sought to investigate the effect and potential intervention mechanism of TMP in anoxia/reoxygenation (A/R) rat hippocampal neurons. MATERIAL AND METHODS After being cultured for 7 days, primary hippocampal neurons were randomly assigned into a normal control group (N), a TMP group (C 0 ug/ml, L 60 ug/ml, M 200ug/ml and H 800 ug/ml), and a JNK inhibitor group (S SP600125, 10 µmol/L). A hypoxia/reoxygenation model were prepared 1 h after incubation. Hippocampal neurons were incubated in 90% N2 and 10% CO2 for 2 h, and then reoxygenated for 24 h in an incubator with 5%CO2 at the temperature of 37°C. The apoptosis rate, MKK4 and MKK7 mRNA and JNK kinase protein levels (C-fos, c-jun, and P-JNK) of hippocampal neurons were detected. RESULTS The apoptosis rates of hippocampal neurons induced by A/R showed significant reduction after being pre-treated with JNK inhibitor, TMP 60 µg/ml, 200 µg/ml, and 800 µg/ml. The JNK kinase MKK4mRNA and MKK7mRNA levels, as well as the expressions of C-fos, C-jun, and P-JNK protein levels, were also be reduced. CONCLUSIONS TMP may produce a protective effect in anoxia/reoxygenation-induced primary hippocampal neuronal injury by inhibiting the apoptosis of the hippocampal neurons; the possible mechanism may be inhibition of the JNK signal pathway.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Oxígeno
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Pirazinas
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Apoptosis
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Fármacos Neuroprotectores
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Quinasas de Proteína Quinasa Activadas por Mitógenos
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Sistema de Señalización de MAP Quinasas
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Hipoxia
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Neuronas
Límite:
Animals
Idioma:
En
Revista:
Med Sci Monit
Asunto de la revista:
MEDICINA
Año:
2016
Tipo del documento:
Article