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Analysis of the alpha-amylase gene of Schwanniomyces occidentalis and the secretion of its gene product in transformants of different yeast genera.
Strasser, A W; Selk, R; Dohmen, R J; Niermann, T; Bielefeld, M; Seeboth, P; Tu, G H; Hollenberg, C P.
Afiliación
  • Strasser AW; Rhein Biotech GmbH, Düsseldorf, Federal Republic of Germany.
Eur J Biochem ; 184(3): 699-706, 1989 Oct 01.
Article en En | MEDLINE | ID: mdl-2806251
We have cloned and characterized the alpha-amylase gene (AMY1) of the yeast Schwanniomyces occidentalis. A cosmid gene library of S. occidentalis DNA was screened in Saccharomyces cerevisiae for alpha-amylase secretion. The positive clone contained a DNA fragment harbouring an open reading frame of 1536 nucleotides coding for a 512-amino-acid polypeptide with a calculated Mr of 56,500. The deduced amino acid sequence reveals significant similarity to the sequence of the Saccharomycopsis fibuligera and Aspergillus oryzae alpha-amylases. The AMY l gene was found to be expressed from its original promoter in S. cerevisiae, Kluyveromyces lactis and Schizo-saccharomyces pombe leading to an active secreted gene product and thus enabling the different yeast transformants to grow on starch as a sole carbon source.
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ascomicetos / Transformación Genética / Levaduras / Alfa-Amilasas Idioma: En Revista: Eur J Biochem Año: 1989 Tipo del documento: Article
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ascomicetos / Transformación Genética / Levaduras / Alfa-Amilasas Idioma: En Revista: Eur J Biochem Año: 1989 Tipo del documento: Article