Fast preparation of a long chimeric armored RNA as controls for external quality assessment for molecular detection of Zika virus.
Clin Chim Acta
; 466: 138-144, 2017 Mar.
Article
en En
| MEDLINE
| ID: mdl-28111270
ABSTRACT
BACKGROUND:
The emergence of Zika virus demands accurate laboratory diagnostics. Nucleic acid testing is currently the definitive method for diagnosis of Zika infection. In 2016, an external quality assurance (EQA) for assessing the quality of molecular testing of Zika virus was carried out in China.METHODS:
A single armored RNA encapsulating a 4942-nucleotides (nt) long specific RNA sequence of Zika virus was prepared and used as positive samples. A pre-tested EQA panel, consisting of 4 negative and 6 positive samples with different concentrations of armored RNA, was distributed to 38 laboratories that perform molecular detection of Zika virus.RESULTS:
A total of 39 data sets (1 laboratory used two test kits in parallel), produced by using commercial (n=38) or laboratory developed (n=1) quantitative reverse-transcriptase PCR (qRT-PCR) kits, were received. Of these, 35 (89.7%) had correct results for all 10 samples, and 4 (10.3%) reported at least 1 error (11 in total). The testing errors were all false-negatives, highlighting the need of improvements in detecting sensitivity.CONCLUSIONS:
The EQA reveals that the majority of participating laboratories are proficient in molecular testing of Zika virus.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ARN Viral
/
Virus Zika
/
Infección por el Virus Zika
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
Clin Chim Acta
Año:
2017
Tipo del documento:
Article