Taming hyperactive hDNase I: Stable inducible expression of a hyperactive salt- and actin-resistant variant of human deoxyribonuclease I in CHO cells.

Lam, Cynthia; Santell, Lydia; Wilson, Blair; Yim, Mandy; Louie, Salina; Tang, Danming; Shaw, David; Chan, Pamela; Lazarus, Robert A; Snedecor, Brad; Misaghi, Shahram

Lam, Cynthia; Santell, Lydia; Wilson, Blair; Yim, Mandy; Louie, Salina; Tang, Danming; Shaw, David; Chan, Pamela; Lazarus, Robert A; Snedecor, Brad; Misaghi, Shahram.

Afiliación

Lam C; Early Stage Cell Culture, Genentech, Inc, South San Francisco, CA, 94080.
Santell L; Early Discovery Biochemistry, Genentech, Inc, South San Francisco, CA, 94080.
Wilson B; Biochemical and Cellular Pharmacology Dept., Genentech, Inc, South San Francisco, CA, 94080.
Yim M; Early Stage Cell Culture, Genentech, Inc, South San Francisco, CA, 94080.
Louie S; Early Stage Cell Culture, Genentech, Inc, South San Francisco, CA, 94080.
Tang D; Early Stage Cell Culture, Genentech, Inc, South San Francisco, CA, 94080.
Shaw D; Early Stage Cell Culture, Genentech, Inc, South San Francisco, CA, 94080.
Chan P; Biochemical and Cellular Pharmacology Dept., Genentech, Inc, South San Francisco, CA, 94080.
Lazarus RA; Early Discovery Biochemistry, Genentech, Inc, South San Francisco, CA, 94080.
Snedecor B; Early Stage Cell Culture, Genentech, Inc, South San Francisco, CA, 94080.
Misaghi S; Early Stage Cell Culture, Genentech, Inc, South San Francisco, CA, 94080.

Biotechnol Prog ; 33(2): 523-533, 2017 03.

Article en En | MEDLINE | ID: mdl-28127892

Asunto(s)

Actinas/química; Clonación Molecular/métodos; Desoxirribonucleasa I/química; Desoxirribonucleasa I/metabolismo; Ingeniería de Proteínas/métodos; Sales (Química)/química; Animales; Células CHO; Proliferación Celular/fisiología; Cricetulus; Desoxirribonucleasa I/genética; Activación Enzimática; Estabilidad de Enzimas; Humanos; Proteínas Recombinantes/química; Proteínas Recombinantes/metabolismo

Palabras clave

cystic fibrosis; hDNase I; hyperactive; inducible expression

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Sales (Química) / Ingeniería de Proteínas / Actinas / Clonación Molecular / Desoxirribonucleasa I Límite: Animals / Humans Idioma: En Revista: Biotechnol Prog Asunto de la revista: BIOTECNOLOGIA Año: 2017 Tipo del documento: Article

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