Direct Cytosolic Delivery of CRISPR/Cas9-Ribonucleoprotein for Efficient Gene Editing.
ACS Nano
; 11(3): 2452-2458, 2017 03 28.
Article
en En
| MEDLINE
| ID: mdl-28129503
ABSTRACT
Genome editing through the delivery of CRISPR/Cas9-ribonucleoprotein (Cas9-RNP) reduces unwanted gene targeting and avoids integrational mutagenesis that can occur through gene delivery strategies. Direct and efficient delivery of Cas9-RNP into the cytosol followed by translocation to the nucleus remains a challenge. Here, we report a remarkably highly efficient (â¼90%) direct cytoplasmic/nuclear delivery of Cas9 protein complexed with a guide RNA (sgRNA) through the coengineering of Cas9 protein and carrier nanoparticles. This construct provides effective (â¼30%) gene editing efficiency and opens up opportunities in studying genome dynamics.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ribonucleoproteínas
/
Técnicas de Transferencia de Gen
/
Citosol
/
Sistemas CRISPR-Cas
/
Edición Génica
Idioma:
En
Revista:
ACS Nano
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos