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PCR-Dipstick Chromatography for Differential Detection of Carbapenemase Genes Directly in Stool Specimens.
Shanmugakani, Rathina Kumar; Akeda, Yukihiro; Yamamoto, Norihisa; Sakamoto, Noriko; Hagiya, Hideharu; Yoshida, Hisao; Takeuchi, Dan; Sugawara, Yo; Kodera, Takuya; Kawase, Mitsuo; Laolerd, Warawut; Chaihongsa, Narong; Santanirand, Pitak; Ishii, Yoshikazu; Hamada, Shigeyuki; Tomono, Kazunori.
Afiliación
  • Shanmugakani RK; Department of Infection Control and Prevention, Graduate School of Medicine, Osaka University, Osaka, Japan.
  • Akeda Y; Division of Infection Control and Prevention, Osaka University Hospital, Osaka, Japan.
  • Yamamoto N; Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
  • Sakamoto N; Department of Infection Control and Prevention, Graduate School of Medicine, Osaka University, Osaka, Japan akeda@biken.osaka-u.ac.jp.
  • Hagiya H; Division of Infection Control and Prevention, Osaka University Hospital, Osaka, Japan.
  • Yoshida H; Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
  • Takeuchi D; Department of Infection Control and Prevention, Graduate School of Medicine, Osaka University, Osaka, Japan.
  • Sugawara Y; Division of Infection Control and Prevention, Osaka University Hospital, Osaka, Japan.
  • Kodera T; Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
  • Kawase M; Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
  • Laolerd W; Department of Infection Control and Prevention, Graduate School of Medicine, Osaka University, Osaka, Japan.
  • Chaihongsa N; Division of Infection Control and Prevention, Osaka University Hospital, Osaka, Japan.
  • Santanirand P; Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
  • Ishii Y; Department of Infection Control and Prevention, Graduate School of Medicine, Osaka University, Osaka, Japan.
  • Hamada S; Division of Infection Control and Prevention, Osaka University Hospital, Osaka, Japan.
  • Tomono K; Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Antimicrob Agents Chemother ; 61(6)2017 06.
Article en En | MEDLINE | ID: mdl-28373197
ABSTRACT
A PCR-dipstick chromatography technique was designed and evaluated for differential identification of blaNDM, blaKPC, blaIMP, and blaOXA-48 carbapenemase genes directly in stool specimens within 2 h. It is a DNA-DNA hybridization-based detection system where PCR products can be easily interpreted by visual observation without electrophoresis. The PCR-dipstick showed high sensitivity (93.3%) and specificity (99.1%) in directly detecting carbapenemase genes in stool specimens compared with multiplex PCR for genomic DNA of the isolates from those stool specimens.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Beta-Lactamasas / Técnicas de Diagnóstico Molecular / Heces / Reacción en Cadena de la Polimerasa Multiplex Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Antimicrob Agents Chemother Año: 2017 Tipo del documento: Article País de afiliación: Japón
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Beta-Lactamasas / Técnicas de Diagnóstico Molecular / Heces / Reacción en Cadena de la Polimerasa Multiplex Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Antimicrob Agents Chemother Año: 2017 Tipo del documento: Article País de afiliación: Japón