PCR-Dipstick Chromatography for Differential Detection of Carbapenemase Genes Directly in Stool Specimens.
Antimicrob Agents Chemother
; 61(6)2017 06.
Article
en En
| MEDLINE
| ID: mdl-28373197
ABSTRACT
A PCR-dipstick chromatography technique was designed and evaluated for differential identification of blaNDM, blaKPC, blaIMP, and blaOXA-48 carbapenemase genes directly in stool specimens within 2 h. It is a DNA-DNA hybridization-based detection system where PCR products can be easily interpreted by visual observation without electrophoresis. The PCR-dipstick showed high sensitivity (93.3%) and specificity (99.1%) in directly detecting carbapenemase genes in stool specimens compared with multiplex PCR for genomic DNA of the isolates from those stool specimens.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Beta-Lactamasas
/
Técnicas de Diagnóstico Molecular
/
Heces
/
Reacción en Cadena de la Polimerasa Multiplex
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Antimicrob Agents Chemother
Año:
2017
Tipo del documento:
Article
País de afiliación:
Japón