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DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells.
Harterink, Martin; da Silva, Marta Esteves; Will, Lena; Turan, Julia; Ibrahim, Adiljan; Lang, Alexander E; van Battum, Eljo Y; Pasterkamp, R Jeroen; Kapitein, Lukas C; Kudryashov, Dmitri; Barres, Ben A; Hoogenraad, Casper C; Zuchero, J Bradley.
Afiliación
  • Harterink M; Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands.
  • da Silva ME; Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands.
  • Will L; Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands.
  • Turan J; Department of Neurobiology, Stanford University School of Medicine, Stanford, California, USA.
  • Ibrahim A; Department of Neurobiology, Stanford University School of Medicine, Stanford, California, USA.
  • Lang AE; Institute for Experimental and Clinical Pharmacology and Toxicology, Albert-Ludwigs University of Freiburg, Freiburg, Germany.
  • van Battum EY; Department of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht, Utrecht, the Netherlands.
  • Pasterkamp RJ; Department of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht, Utrecht, the Netherlands.
  • Kapitein LC; Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands.
  • Kudryashov D; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio, USA.
  • Barres BA; Department of Neurobiology, Stanford University School of Medicine, Stanford, California, USA.
  • Hoogenraad CC; Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands.
  • Zuchero JB; Department of Neurobiology, Stanford University School of Medicine, Stanford, California, USA.
Nat Methods ; 14(5): 479-482, 2017 May.
Article en En | MEDLINE | ID: mdl-28394337
ABSTRACT
The actin cytoskeleton is essential for many fundamental biological processes, but tools for directly manipulating actin dynamics are limited to cell-permeable drugs that preclude single-cell perturbations. Here we describe DeActs, genetically encoded actin-modifying polypeptides, which effectively induce actin disassembly in eukaryotic cells. We demonstrate that DeActs are universal tools for studying the actin cytoskeleton in single cells in culture, tissues, and multicellular organisms including various neurodevelopmental model systems.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Péptidos / Proteínas Recombinantes de Fusión / Citoesqueleto de Actina / Actinas / ADP Ribosa Transferasas / Gelsolina / Factores de Virulencia Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2017 Tipo del documento: Article País de afiliación: Países Bajos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Péptidos / Proteínas Recombinantes de Fusión / Citoesqueleto de Actina / Actinas / ADP Ribosa Transferasas / Gelsolina / Factores de Virulencia Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2017 Tipo del documento: Article País de afiliación: Países Bajos