Iterative expansion microscopy.
Nat Methods
; 14(6): 593-599, 2017 Jun.
Article
en En
| MEDLINE
| ID: mdl-28417997
ABSTRACT
We recently developed a method called expansion microscopy, in which preserved biological specimens are physically magnified by embedding them in a densely crosslinked polyelectrolyte gel, anchoring key labels or biomolecules to the gel, mechanically homogenizing the specimen, and then swelling the gel-specimen composite by â¼4.5× in linear dimension. Here we describe iterative expansion microscopy (iExM), in which a sample is expanded â¼20×. After preliminary expansion a second swellable polymer mesh is formed in the space newly opened up by the first expansion, and the sample is expanded again. iExM expands biological specimens â¼4.5 × 4.5, or â¼20×, and enables â¼25-nm-resolution imaging of cells and tissues on conventional microscopes. We used iExM to visualize synaptic proteins, as well as the detailed architecture of dendritic spines, in mouse brain circuitry.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Polímeros
/
Manejo de Especímenes
/
Aumento de la Imagen
/
Micromanipulación
/
Microscopía
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos