A method for the detection of virus infectivity in single cells and real time: Towards an automated fluorescence neutralization test.
Virus Res
; 237: 1-6, 2017 06 02.
Article
en En
| MEDLINE
| ID: mdl-28501626
ABSTRACT
Virus neutralizing antibodies are critical correlates of protection in vaccine development and are discriminatory in the plaque reduction neutralization test when used for the diagnosis of viral infections. However, neutralization assays are time consuming, labor intensive and highly variable, thus limiting their use. Advances in automated live imaging of cells opened new possibilities for standard virus diagnostic techniques such as neutralization assays. To this end, a reporter cell line based on the translocation of the transcription factor IRF3 in response to infection is proposed. Image acquisition of signal in a microplate format allowed the setup of a rapid, semi-automated and high-throughput fluorescent neutralization test. The study is extended to the live imaging of IRF3 translocations that could potentially cut the time of analysis to few hours. The fluorescent neutralization test is suitable for high-throughput assays and expandable to other viruses of global importance such as Zika virus.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Pruebas de Neutralización
/
Automatización de Laboratorios
/
Análisis de la Célula Individual
/
Fluorometría
/
Microscopía Intravital
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Virus Res
Asunto de la revista:
VIROLOGIA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Italia