Novel Approaches to Analyze Immunoglobulin Repertoires.
Trends Immunol
; 38(7): 471-482, 2017 07.
Article
en En
| MEDLINE
| ID: mdl-28566130
ABSTRACT
Analysis of immunoglobulin (Ig) repertoires aims to comprehend Ig diversity with the goal of predicting humoral immune responses in the context of infection, vaccination, autoimmunity, and malignancies. The first next-generation sequencing (NGS) analyses of bulk B cell populations dramatically advanced sampling depth over previous low-throughput single-cell-based protocols, albeit at the expense of accuracy and loss of chain-pairing information. In recent years the field has substantially differentiated, with bulk analyses becoming more accurate while single-cell approaches have gained in throughput. Additionally, new platforms striving to combine high throughput and chain pairing have been developed as well as various computational tools for analysis. Here we review the developments of the past 4-5 years and discuss the open challenges.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Genes de Inmunoglobulinas
/
Linfocitos B
/
Enfermedades Transmisibles
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Cambio de Clase de Inmunoglobulina
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Inmunidad Humoral
/
Anticuerpos
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Trends Immunol
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
2017
Tipo del documento:
Article