Cutaneous Alpha-Synuclein From Paraffin Embedded Autopsy Specimens in Parkinson's Disease.
J Parkinsons Dis
; 7(3): 503-509, 2017.
Article
en En
| MEDLINE
| ID: mdl-28582870
ABSTRACT
BACKGROUND:
Parkinson disease (PD) is neurodegenerative disorder characterized by tremor, rigidity and bradykinesia and pathologically by the deposition of alpha-synuclein within different tissues. We, and others, have reported the detection of cutaneous alpha-synuclein in individuals with PD.OBJECTIVE:
The goal of the present study was to detect alpha-synuclein deposition by immunohistochemical staining of skin samples in pathologically confirmed cases of PD.METHODS:
Post-mortem skin biopsy samples from 11 individuals with PD, and 5 non-synucleinopathy control subjects were paraffin embedded and stained for total alpha-synuclein and protein gene product 9.5.RESULTS:
Alpha-synuclein deposition was greater in both scalp and abdominal skin biopsy PD samples compared to control samples in pilomotor nerves (Pâ<â0.05), sudomotor nerves (Pâ<â0.05) and vasomotor nerves (Pâ<â0.05). Deposition of alpha-synuclein in scalp and abdominal tissue did not correlate with age, duration of PD, or severity of PD.CONCLUSIONS:
There is greater deposition of alpha-synuclein within pilomotor, sudomotor and vasomotor nerve fibers of paraffin embedded samples from autopsy confirmed cases of PD compared to control samples. However, assessment of alpha-synuclein deposition in post-mortem paraffin embedded tissue has many limitations and the utility of this technique in clinical and research studies is uncertain.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Enfermedad de Parkinson
/
Piel
/
Autopsia
/
Alfa-Sinucleína
Límite:
Aged
/
Aged80
/
Humans
Idioma:
En
Revista:
J Parkinsons Dis
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos