Unique Organization of the Nuclear Envelope in the Post-natal Quiescent Neural Stem Cells.
Stem Cell Reports
; 9(1): 203-216, 2017 07 11.
Article
en En
| MEDLINE
| ID: mdl-28648897
ABSTRACT
Neural stem cells (B1 astrocytes; NSCs) in the adult ventricular-subventricular-zone (V-SVZ) originate in the embryo. Surprisingly, recent work has shown that B1 cells remain largely quiescent. They are reactivated postnatally to function as primary progenitors for neurons destined for the olfactory bulb and some corpus callosum oligodendrocytes. The cellular and molecular properties of quiescent B1 cells remain unknown. Here we found that a subpopulation of B1 cells has a unique nuclear envelope invagination specialization similar to envelope-limited chromatin sheets (ELCS), reported in certain lymphocytes and some cancer cells. Using molecular markers, [3H]thymidine birth-dating, and Ara-C, we found that B1 cells with ELCS correspond to quiescent NSCs. ELCS begin forming in embryonic radial glia cells and represent a specific nuclear compartment containing particular epigenetic modifications and telomeres. These results reveal a unique nuclear compartment in quiescent NSCs, which is useful for identifying these primary progenitors and study their gene regulation.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ventrículos Laterales
/
Células-Madre Neurales
/
Membrana Nuclear
Límite:
Animals
Idioma:
En
Revista:
Stem Cell Reports
Año:
2017
Tipo del documento:
Article
País de afiliación:
España