Drosophila CP190- and dCTCF-mediated enhancer blocking is augmented by SUMOylation.
Epigenetics Chromatin
; 10: 32, 2017.
Article
en En
| MEDLINE
| ID: mdl-28680483
ABSTRACT
BACKGROUND:
Chromatin insulators shield promoters and chromatin domains from neighboring enhancers or chromatin regions with opposing activities. Insulator-binding proteins and their cofactors mediate the boundary function. In general, covalent modification of proteins by the small ubiquitin-like modifier (SUMO) is an important mechanism to control the interaction of proteins within complexes.RESULTS:
Here we addressed the impact of dSUMO in respect of insulator function, chromatin binding of insulator factors and formation of insulator speckles in Drosophila. SUMOylation augments the enhancer blocking function of four different insulator sequences and increases the genome-wide binding of the insulator cofactor CP190.CONCLUSIONS:
These results indicate that enhanced chromatin binding of SUMOylated CP190 causes fusion of insulator speckles, which may allow for more efficient insulation.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Represoras
/
Proteínas Nucleares
/
Elementos de Facilitación Genéticos
/
Proteínas de Drosophila
/
Drosophila
/
Sumoilación
/
Proteínas Asociadas a Microtúbulos
Límite:
Animals
Idioma:
En
Revista:
Epigenetics Chromatin
Año:
2017
Tipo del documento:
Article
País de afiliación:
Alemania