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Amphotericin B induces apoptosis-like programmed cell death in Naegleria fowleri and Naegleria gruberi.
Cárdenas-Zúñiga, Roberto; Silva-Olivares, Angélica; Villalba-Magdaleno, José D' Artagnan; Sánchez-Monroy, Virginia; Serrano-Luna, Jesús; Shibayama, Mineko.
Afiliación
  • Cárdenas-Zúñiga R; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute, Av. IPN 2508, San Pedro Zacatenco, 07360, Mexico City, Mexico.
  • Silva-Olivares A; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute, Av. IPN 2508, San Pedro Zacatenco, 07360, Mexico City, Mexico.
  • Villalba-Magdaleno JA; Universidad del Valle de México, Av. Observatorio 400, Col. 16 de Septiembre, 11810, Mexico City, Mexico.
  • Sánchez-Monroy V; Laboratorio de Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía, Instituto Politénico Nacional, Calle Guillermo Massieu H. 239, Col. La Escalera, 07320, Mexico City, Mexico.
  • Serrano-Luna J; Department of Cell Biology, Center for Research and Advanced Studies of the National Polytechnic Institute, Av. IPN 2508, San Pedro Zacatenco, 07360, Mexico City, Mexico.
  • Shibayama M; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute, Av. IPN 2508, San Pedro Zacatenco, 07360, Mexico City, Mexico.
Microbiology (Reading) ; 163(7): 940-949, 2017 07.
Article en En | MEDLINE | ID: mdl-28721850
Naegleria fowleri and Naegleria gruberi belong to the free-living amoebae group. It is widely known that the non-pathogenic species N. gruberi is usually employed as a model to describe molecular pathways in this genus, mainly because its genome has been recently described. However, N. fowleri is an aetiological agent of primary amoebic meningoencephalitis, an acute and fatal disease. Currently, the most widely used drug for its treatment is amphotericin B (AmB). It was previously reported that AmB has an amoebicidal effect in both N. fowleri and N. gruberi trophozoites by inducing morphological changes that resemble programmed cell death (PCD). PCD is a mechanism that activates morphological, biochemical and genetic changes. However, PCD has not yet been characterized in the genus Naegleria. The aim of the present work was to evaluate the typical markers to describe PCD in both amoebae. These results showed that treated trophozoites displayed several parameters of apoptosis-like PCD in both species. We observed ultrastructural changes, an increase in reactive oxygen species, phosphatidylserine externalization and a decrease in intracellular potassium, while DNA degradation was evaluated using the TUNEL assay and agarose gels, and all of these parameters are related to PCD. Finally, we analysed the expression of apoptosis-related genes, such as sir2 and atg8, in N. gruberi. Taken together, our results showed that AmB induces the morphological, biochemical and genetic changes of apoptosis-like PCD in the genus Naegleria.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Naegleria / Anfotericina B / Naegleria fowleri / Apoptosis / Infecciones Protozoarias del Sistema Nervioso Central / Antiprotozoarios Idioma: En Revista: Microbiology (Reading) Asunto de la revista: MICROBIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: México

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Naegleria / Anfotericina B / Naegleria fowleri / Apoptosis / Infecciones Protozoarias del Sistema Nervioso Central / Antiprotozoarios Idioma: En Revista: Microbiology (Reading) Asunto de la revista: MICROBIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: México