Genomic characterization of a novel calicivirus, FHMCV-2012, from baitfish in the USA.
Arch Virol
; 162(12): 3619-3627, 2017 Dec.
Article
en En
| MEDLINE
| ID: mdl-28815386
ABSTRACT
During regulatory sampling of fathead minnows (Pimephales promelas), a novel calicivirus was isolated from homogenates of kidney and spleen inoculated into bluegill fry (BF-2) cells. Infected cell cultures exhibiting cytopathic effects were screened by PCR-based methods for selected fish viral pathogens. Illumina HiSeq next generation sequencing of the total RNA revealed a novel calicivirus genome that showed limited protein sequence similarity to known homologs in a BLASTp search. The complete genome of this fathead minnow calicivirus (FHMCV) is 6564 nt long, encoding a polyprotein of 2114 aa in length. The complete polyprotein shared only 21% identity with Atlantic salmon calicivirus,followed by 11% to 14% identity with mammalian caliciviruses. A molecular detection assay (RT-PCR) was designed from this sequence for screening of field samples for FHMCV in the future. This virus likely represents a prototype species of a novel genus in the family Caliciviridae, tentatively named "Minovirus".
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Filogenia
/
Caliciviridae
/
Cyprinidae
/
Genoma Viral
/
Infecciones por Caliciviridae
Límite:
Animals
País/Región como asunto:
America do norte
Idioma:
En
Revista:
Arch Virol
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos