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Genomic characterization of a novel calicivirus, FHMCV-2012, from baitfish in the USA.
Mor, Sunil Kumar; Phelps, Nicholas B D; Ng, Terry Fei Fan; Subramaniam, Kuttichantran; Primus, Alexander; Armien, Anibal G; McCann, Rebekah; Puzach, Corey; Waltzek, Thomas B; Goyal, Sagar M.
Afiliación
  • Mor SK; Minnesota Veterinary Diagnostic Laboratory, Department of Veterinary Population Medicine, University of Minnesota, 1333 Gortner Avenue, St. Paul, MN, 55108, USA. kumars@umn.edu.
  • Phelps NBD; Minnesota Aquatic Invasive Species Research Center, Department of Fisheries, Wildlife, and Conservation Biology, College of Food, Agriculture, and Natural Resource Sciences, University of Minnesota, St. Paul, MN, 55108, USA.
  • Ng TFF; College of Veterinary Medicine, University of Georgia, 501 D. W. Brooks Drive, Athens, GA, 30602, USA.
  • Subramaniam K; Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL, 32610, USA.
  • Primus A; Minnesota Veterinary Diagnostic Laboratory, Department of Veterinary Population Medicine, University of Minnesota, 1333 Gortner Avenue, St. Paul, MN, 55108, USA.
  • Armien AG; Minnesota Veterinary Diagnostic Laboratory, Department of Veterinary Population Medicine, University of Minnesota, 1333 Gortner Avenue, St. Paul, MN, 55108, USA.
  • McCann R; US Fish and Wildlife Service, La Crosse Fish Health Center, 555 Lester Avenue, Onalaska, WI, 54650, USA.
  • Puzach C; US Fish and Wildlife Service, La Crosse Fish Health Center, 555 Lester Avenue, Onalaska, WI, 54650, USA.
  • Waltzek TB; Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL, 32610, USA.
  • Goyal SM; Minnesota Veterinary Diagnostic Laboratory, Department of Veterinary Population Medicine, University of Minnesota, 1333 Gortner Avenue, St. Paul, MN, 55108, USA.
Arch Virol ; 162(12): 3619-3627, 2017 Dec.
Article en En | MEDLINE | ID: mdl-28815386
ABSTRACT
During regulatory sampling of fathead minnows (Pimephales promelas), a novel calicivirus was isolated from homogenates of kidney and spleen inoculated into bluegill fry (BF-2) cells. Infected cell cultures exhibiting cytopathic effects were screened by PCR-based methods for selected fish viral pathogens. Illumina HiSeq next generation sequencing of the total RNA revealed a novel calicivirus genome that showed limited protein sequence similarity to known homologs in a BLASTp search. The complete genome of this fathead minnow calicivirus (FHMCV) is 6564 nt long, encoding a polyprotein of 2114 aa in length. The complete polyprotein shared only 21% identity with Atlantic salmon calicivirus,followed by 11% to 14% identity with mammalian caliciviruses. A molecular detection assay (RT-PCR) was designed from this sequence for screening of field samples for FHMCV in the future. This virus likely represents a prototype species of a novel genus in the family Caliciviridae, tentatively named "Minovirus".
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Filogenia / Caliciviridae / Cyprinidae / Genoma Viral / Infecciones por Caliciviridae Límite: Animals País/Región como asunto: America do norte Idioma: En Revista: Arch Virol Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Filogenia / Caliciviridae / Cyprinidae / Genoma Viral / Infecciones por Caliciviridae Límite: Animals País/Región como asunto: America do norte Idioma: En Revista: Arch Virol Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos