Correlation of c-erbB-2 gene amplification and protein expression in human breast carcinoma with nodal status and nuclear grading.
Cancer Res
; 48(5): 1238-43, 1988 Mar 01.
Article
en En
| MEDLINE
| ID: mdl-2893663
ABSTRACT
Fifty-one primary human breast tumors were analyzed for amplification of the c-erbB-2 protooncogene. Thirteen (25%) of the DNA samples contained multiple gene copies. Paraffin-embedded tumor sections, available from 47 of the cases, were stained with a c-erbB-2 specific antiserum. Eighty-three % (10 of 12) of the tumors containing amplified c-erbB-2 gene copies stained positively with the c-erbB-2 specific antiserum (P = 0.03). Thirteen tumors containing single copy c-erbB-2 sequences also stained positively with the antiserum. This suggests that mechanisms other than gene amplification may lead to elevated levels of c-erbB-2 protein. Finally, there was a statistically significant correlation between c-erbB-2 protein expression and parameters used in breast cancer prognosis. Positive staining was associated with positive nodal status of the patient (P = 0.02) and with tumors showing a poor nuclear grade (P = 0.02). This is the first study showing that a determination of the level of c-erbB-2 protein in paraffin-embedded tumor sections may have prognostic value for the course of human breast cancer.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proto-Oncogenes
/
Neoplasias de la Mama
/
Amplificación de Genes
/
Proteínas Proto-Oncogénicas
/
Ganglios Linfáticos
Límite:
Female
/
Humans
Idioma:
En
Revista:
Cancer Res
Año:
1988
Tipo del documento:
Article
País de afiliación:
Reino Unido