Novel cell surface luciferase reporter for high-throughput yeast one-hybrid screens.
Nucleic Acids Res
; 45(18): e157, 2017 Oct 13.
Article
en En
| MEDLINE
| ID: mdl-28985361
ABSTRACT
Gene-centered yeast one-hybrid (Y1H) screens provide a powerful and effective strategy to identify transcription factor (TF)-promoter interactions. While genome-wide TF ORFeome clone collections are increasingly available, screening protocols have limitations inherent to the properties of the enzymatic reaction used to identify interactions and to the procedure required to perform the assay in a high-throughput format. Here, we present the development and validation of a streamlined strategy for quantitative and fully automated gene-centered Y1H screens using a novel cell surface Gaussia luciferase reporter.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Genes Reporteros
/
Técnicas del Sistema de Dos Híbridos
/
Ensayos Analíticos de Alto Rendimiento
/
Luciferasas
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Nucleic Acids Res
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos