Yeast phospholipase C is required for stability of casein kinase I Yck2p and expression of hexose transporters.
FEMS Microbiol Lett
; 364(22)2017 Dec 01.
Article
en En
| MEDLINE
| ID: mdl-29087456
ABSTRACT
Phospholipase C (Plc1p) in Saccharomyces cerevisiae is required for normal degradation of repressor Mth1p and expression of the HXT genes encoding cell membrane transporters of glucose. Plc1p is also required for normal localization of glucose transporters to the cell membrane. Consequently, plc1Δ cells display histone hypoacetylation and transcriptional defects due to reduced uptake and metabolism of glucose to acetyl-CoA, a substrate for histone acetyltransferases. In the presence of glucose, Mth1p is phosphorylated by casein kinase I Yck1/2p, ubiquitinated by the SCFGrr1 complex and degraded by the proteasome. Here, we show that while Plc1p does not affect the function of the SCFGrr1 complex or the proteasome, it is required for normal protein level of Yck2p. Since stability of Yck1/2p is regulated by a glucose-dependent mechanism, PLC1 inactivation results in destabilization of Yck1/2p and defect in Mth1p degradation. Based on our results and published data, we propose a model in which plc1Δ mutation causes increased internalization of glucose transporters, decreased transport of glucose into the cells, and consequently decreased stability of Yck1/2p, increased stability of Mth1p and decreased expression of the HXT genes.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fosfolipasas de Tipo C
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Proteínas de Transporte de Monosacáridos
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Proteínas Recombinantes
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Proteínas de Saccharomyces cerevisiae
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Quinasa de la Caseína I
Idioma:
En
Revista:
FEMS Microbiol Lett
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos