The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein.
Science
; 230(4732): 1344-9, 1985 Dec 20.
Article
en En
| MEDLINE
| ID: mdl-2933810
A component present in splicing extracts selectively binds the 3' splice site of a precursor messenger RNA (pre-mRNA) transcript of a human beta-globin gene. Since this component can be immunoprecipitated by either autoantibodies of the Sm class or antibodies specifically directed against trimethylguanosine, it is a small nuclear ribonucleoprotein (snRNP). Its interaction with the 3' splice site occurs rapidly even at 0 degrees C, does not require adenosine triphosphate, and is altered by certain mutations in the 3' splice site region. Binding is surprisingly insensitive to treatment of the extract with micrococcal nuclease. The U5 particle is the only abundant Sm snRNP with a capped 5' end that is equally resistant to micrococcal nuclease. This suggests that, in addition to the U1 and U2 snRNP's, U5 snRNP's participate in pre-mRNA splicing.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ribonucleoproteínas
/
ARN Mensajero
/
Empalme del ARN
/
Precursores de Ácido Nucleico
Límite:
Humans
Idioma:
En
Revista:
Science
Año:
1985
Tipo del documento:
Article