Spermicidal Constituents of Ethanolic Extract of Sacoglottis gabonensis Stem Bark.
Folia Med (Plovdiv)
; 59(4): 437-442, 2017 Dec 20.
Article
en En
| MEDLINE
| ID: mdl-29341951
ABSTRACT
AIM:
To isolate the spermicidal constituents of Sacoglottis gabonensis. MATERIALS ANDMETHODS:
The ethanolic extract with partitioned fractions of Sacoglottis gabonensis stem bark were subjected to sperm immobilization assay. The most active EtOAc fraction was further purifi ed by column and Semi-Preparative High Performance Liquid Chromatography to give compounds which were characterized by spectroscopic methods (UV, LC/MS, and NMR). The compound(s) was also tested for sperm immobilization activity.RESULTS:
The ethanolic extract showed 100% signifi cant (p < 0.05) sperm immobilization activity at a concentration of 30 mg/mL at 20 s compared to both negative and positive controls. The most active ethyl acetate fraction yielded methyl 3,5-dihydroxy-4-methoxybenzoate, eriodictyol and bergenin. Bergenin had 100% sperm immobilization activity at 20 mg/mL in 60 s which was signifi cant (p < 0.05) also when compared to the positive and negative control while methyl 3,5-dihydroxy- 4-methoxybenzoate, eriodictyol were not active.CONCLUSION:
The active spermicidal constituent in Sacoglottis gabonensis stem bark extract is bergenin. However, methyl 3,5-dihydroxy-4-methoxybenzoate and eriodictyol showed no activity. This plant is known for its aphrodisiac action; hence, caution may have to be exercised in its use because of its spermicidal eff ect.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Espermicidas
/
Extractos Vegetales
/
Magnoliopsida
Límite:
Animals
Idioma:
En
Revista:
Folia Med (Plovdiv)
Año:
2017
Tipo del documento:
Article
País de afiliación:
Nigeria