Paeoniflorin induces G2/M cell cycle arrest and caspase-dependent apoptosis through the upregulation of Bcl-2 X-associated protein and downregulation of B-cell lymphoma 2 in human osteosarcoma cells.
Mol Med Rep
; 17(4): 5095-5101, 2018 04.
Article
en En
| MEDLINE
| ID: mdl-29363721
ABSTRACT
Paeoniflorin (PF), extracted from the peony root, has been proved to possess antineoplastic activity in different cancer cell lines. However, it remains unclear whether PF has an antineoplastic effect against osteosarcoma cells. The present study investigated the effects and the specific mechanism of PF on various human osteosarcoma cell lines. Using the multiple methods to detect the activity of PF on HOS and Saos2 human osteosarcoma cell lines, including an MTS assay, flow cytometry, transmission electron microscopy and western blotting, it was demonstrated that PF induces inhibition of proliferation, G2/M phase cell cycle arrest and apoptosis in the osteosarcoma cell lines in vitro, and activation of cleavedcaspase3 and cleavedpoly (ADPribose) polymerase in a dosedependent manner. Furthermore, the proapoptotic factors Bcl2 Xassociated protein and BH3 interacting domain death agonist were uregulated, while the antiapoptotic factors Bcell lymphoma 2 (Bcl2) and Bcl2extra large were downregulated. In conclusion, these results demonstrated that PF has a promising therapeutic potential in for osteosarcoma.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Apoptosis
/
Proteínas Proto-Oncogénicas c-bcl-2
/
Caspasas
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Monoterpenos
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Proteína X Asociada a bcl-2
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Puntos de Control de la Fase G2 del Ciclo Celular
/
Glucósidos
Tipo de estudio:
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Mol Med Rep
Año:
2018
Tipo del documento:
Article