Using xCELLigence RTCA Instrument to Measure Cell Adhesion.
Bio Protoc
; 7(24)2017 Dec 20.
Article
en En
| MEDLINE
| ID: mdl-29367941
ABSTRACT
Cell adhesion to neighbouring cells and to the underlying extracellular matrix (ECM) is a fundamental requirement for the existence of multicellular organisms. As such, the formation, stability and dissociation of cell adhesions are subject to tight control in space and time and perturbations within the sophisticated adhesion machinery are associated with a variety of human pathologies. Here, we outline a simple protocol to monitor alterations in cell adhesion to the ECM, for example, following genetic manipulations or overexpression of a protein of interest or in response to drug treatment, using the xCELLigence real-time cell analysis (RTCA) system.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Tipo de estudio:
Guideline
Idioma:
En
Revista:
Bio Protoc
Año:
2017
Tipo del documento:
Article
País de afiliación:
Finlandia