[Determination of Hair Shafts by InnoTyper® 21 Kit].
Fa Yi Xue Za Zhi
; 33(6): 615-618, 2017 Dec.
Article
en Zh
| MEDLINE
| ID: mdl-29441770
ABSTRACT
OBJECTIVES:
To explore the application value of InnoTyper® 21 kit in forensic practice.METHODS:
Samples of hair shafts and saliva were collected from 8 unrelated individuals. Template DNA was extracted by AutoMate Express™ forensic DNA automatic extraction system. DNA was amplified by InnoTyper® 21 kit and AmpFâSTR™ Identifiler™ Plus kit, respectively, and then the results were compared.RESULTS:
After the amplification by InnoTyper® 21 kit, complete specific genotyping could be detected from the saliva samples, and the peak value of genotyping profiles of hair shafts without sheath cells was 57-1 219 RFU. Allelic gene deletion could be found sometimes. When amplified by AmpFâSTR™ Identifiler™ Plus kit, complete specific genotyping could be detected from the saliva samples, and the specific fragment was not detected in hair shafts without sheath cells.CONCLUSIONS:
The InnoTyper® 21 kit has certain application value in the cases of hair shafts without sheath cells.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Saliva
/
ADN
/
Dermatoglifia del ADN
/
Cabello
Límite:
Humans
Idioma:
Zh
Revista:
Fa Yi Xue Za Zhi
Asunto de la revista:
JURISPRUDENCIA
Año:
2017
Tipo del documento:
Article
País de afiliación:
China