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Recognition of RNA N6-methyladenosine by IGF2BP proteins enhances mRNA stability and translation.
Huang, Huilin; Weng, Hengyou; Sun, Wenju; Qin, Xi; Shi, Hailing; Wu, Huizhe; Zhao, Boxuan Simen; Mesquita, Ana; Liu, Chang; Yuan, Celvie L; Hu, Yueh-Chiang; Hüttelmaier, Stefan; Skibbe, Jennifer R; Su, Rui; Deng, Xiaolan; Dong, Lei; Sun, Miao; Li, Chenying; Nachtergaele, Sigrid; Wang, Yungui; Hu, Chao; Ferchen, Kyle; Greis, Kenneth D; Jiang, Xi; Wei, Minjie; Qu, Lianghu; Guan, Jun-Lin; He, Chuan; Yang, Jianhua; Chen, Jianjun.
Afiliación
  • Huang H; Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
  • Weng H; Department of Systems Biology, City of Hope, Monrovia, CA, USA.
  • Sun W; Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
  • Qin X; Department of Systems Biology, City of Hope, Monrovia, CA, USA.
  • Shi H; Key Laboratory of Gene Engineering of the Ministry of Education, Sun Yat-sen University, Guangzhou, China.
  • Wu H; State Key Laboratory for Biocontrol, Sun Yat-sen University, Guangzhou, China.
  • Zhao BS; Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
  • Mesquita A; Department of Systems Biology, City of Hope, Monrovia, CA, USA.
  • Liu C; Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL, USA.
  • Yuan CL; Howard Hughes Medical Institute, The University of Chicago, Chicago, IL, USA.
  • Hu YC; Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
  • Hüttelmaier S; Department of Systems Biology, City of Hope, Monrovia, CA, USA.
  • Skibbe JR; Department of Pharmacology, School of Pharmacy, China Medical University, Shenyang, China.
  • Su R; Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL, USA.
  • Deng X; Howard Hughes Medical Institute, The University of Chicago, Chicago, IL, USA.
  • Dong L; Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
  • Sun M; Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL, USA.
  • Li C; Howard Hughes Medical Institute, The University of Chicago, Chicago, IL, USA.
  • Nachtergaele S; Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
  • Wang Y; Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
  • Hu C; Institute of Molecular Medicine, Department of Molecular Cell Biology, Martin Luther University, Halle, Germany.
  • Ferchen K; Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
  • Greis KD; Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
  • Jiang X; Department of Systems Biology, City of Hope, Monrovia, CA, USA.
  • Wei M; Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
  • Qu L; Department of Systems Biology, City of Hope, Monrovia, CA, USA.
  • Guan JL; Department of Pharmacology, School of Pharmacy, China Medical University, Shenyang, China.
  • He C; Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
  • Yang J; Department of Systems Biology, City of Hope, Monrovia, CA, USA.
  • Chen J; Division of Human Genetics, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
Nat Cell Biol ; 20(3): 285-295, 2018 03.
Article en En | MEDLINE | ID: mdl-29476152
ABSTRACT
N6-methyladenosine (m6A) is the most prevalent modification in eukaryotic messenger RNAs (mRNAs) and is interpreted by its readers, such as YTH domain-containing proteins, to regulate mRNA fate. Here, we report the insulin-like growth factor 2 mRNA-binding proteins (IGF2BPs; including IGF2BP1/2/3) as a distinct family of m6A readers that target thousands of mRNA transcripts through recognizing the consensus GG(m6A)C sequence. In contrast to the mRNA-decay-promoting function of YTH domain-containing family protein 2, IGF2BPs promote the stability and storage of their target mRNAs (for example, MYC) in an m6A-dependent manner under normal and stress conditions and therefore affect gene expression output. Moreover, the K homology domains of IGF2BPs are required for their recognition of m6A and are critical for their oncogenic functions. Thus, our work reveals a different facet of the m6A-reading process that promotes mRNA stability and translation, and highlights the functional importance of IGF2BPs as m6A readers in post-transcriptional gene regulation and cancer biology.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: ARN Mensajero / Adenosina / Procesamiento Postranscripcional del ARN / Proteínas de Unión al ARN / Estabilidad del ARN Límite: Female / Humans Idioma: En Revista: Nat Cell Biol Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: ARN Mensajero / Adenosina / Procesamiento Postranscripcional del ARN / Proteínas de Unión al ARN / Estabilidad del ARN Límite: Female / Humans Idioma: En Revista: Nat Cell Biol Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos