Urinary afamin levels are associated with the progression of diabetic nephropathy.
Diabetes Res Clin Pract
; 147: 37-46, 2019 Jan.
Article
en En
| MEDLINE
| ID: mdl-29522788
ABSTRACT
AIMS:
In this study, we applied quantitative proteomic analysis to identify urinary proteins associated with diabetic nephropathy (DN).METHODS:
Two-dimensional image-converted analysis of liquid chromatography and mass spectrometry detected the proteins differentially excreted between normoalbuminuric and macroalbuminuric patients with type 2 diabetes mellitus (T2DM) (nâ¯=â¯6 each). Urinary levels of excreted proteins were measured by multiple reaction monitoring (MRM) analysis using an independent sample set (nâ¯=â¯77). Urinary afamin levels were measured by ELISA in T2DM and DN patients enrolled in this cohort study (nâ¯=â¯203).RESULTS:
One-hundred-four proteins displayed significant alterations in excretion. Nine of these candidates were validated by MRM analysis. Among them, the levels of afamin, CD44 antigen, and lysosome-associated membrane glycoprotein 2, which have not previously been implicated in DN, were significantly associated with both the urinary albumin to creatinine ratio (ACR) and eGFR. We further measured afamin levels in urine collected from T2DM patients who did not yet have significant kidney disease (ACRâ¯<â¯300â¯mg/g or eGFR change rateâ¯≤â¯3.3%/year). The urinary afamin to creatinine ratio (Afa/Cre) was significantly higher in patients who progressed to a more severe DN stage or had early renal decline than in patients who did not.CONCLUSIONS:
Afa/Cre was significantly increased in T2DM patients who subsequently developed DN. Afa/Cre may be useful to predict patients with T2DM at high risk of nephropathy before the development of macroalbuminuria or reduced kidney function, although further validation studies in a larger population are needed.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Glicoproteínas
/
Proteínas Portadoras
/
Proteómica
/
Diabetes Mellitus Tipo 2
/
Nefropatías Diabéticas
/
Albúmina Sérica Humana
Tipo de estudio:
Etiology_studies
/
Incidence_studies
/
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Límite:
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Diabetes Res Clin Pract
Asunto de la revista:
ENDOCRINOLOGIA
Año:
2019
Tipo del documento:
Article
País de afiliación:
Japón