miR-205-5p negatively regulates hepatic acetyl-CoA carboxylase ß mRNA in lipid metabolism of Oreochromis niloticus.
Gene
; 660: 1-7, 2018 Jun 20.
Article
en En
| MEDLINE
| ID: mdl-29574186
ABSTRACT
MicroRNAs (miRNAs) are non-coding RNAs that function as post-transcriptional gene regulators and that play vital roles controlling lipid metabolism. miR-205 is an important miRNA related to adipogenesis and lipid metabolism. However, little is known about the potential role of miR-205-5p in genetically improved farmed tilapia (GIFT, Oreochromis niloticus). In this study, we used miRanda software to search for potential miR-205-5p target genes and found a lipid-metabolism-related gene called acetyl-CoA carboxylase ß (ACACß). Quantitative real-time polymerase chain reaction data indicated that there may be a negative regulation relationship between miR-205-5p and ACACß gene expression under HFD rearing. Using luciferase reporter assays, we verified the binding site of miR-205-5p in the 3'-untranslated region of the ACACß mRNA. Furthermore, an in vivo functional analysis of miR-205-5p was performed by injecting GIFT juveniles with a miR-205-5p antagomir. Reduced levels of miR-205-5p in GIFT liver increased ACACß mRNA expression 12â¯h post-injection. miR-205-5p suppression also increased fatty acid synthase and peroxisome proliferator-activated receptor-α mRNA levels 48â¯h and 120â¯h post-injection, respectively. Taken together, our results indicate that miR-205-5p negative regulates hepatic ACACß mRNA expression, and may serve as an important regulator in controlling hepatic lipid metabolism in GIFT.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Acetil-CoA Carboxilasa
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ARN Mensajero
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Cíclidos
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Proteínas de Peces
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MicroARNs
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Metabolismo de los Lípidos
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Hígado
Límite:
Animals
Idioma:
En
Revista:
Gene
Año:
2018
Tipo del documento:
Article
País de afiliación:
China