Anthricininduced caspasedependent apoptosis through IGF1R/PI3K/AKT pathway inhibition in A549 human nonsmall lung cancer cells.
Oncol Rep
; 39(6): 2769-2776, 2018 Jun.
Article
en En
| MEDLINE
| ID: mdl-29620219
ABSTRACT
Anthricin (deoxypodophyllotoxin) is a major lignan in Anthriscus sylvestris and possesses many bioactivities such as antiproliferative, antitumor, antiplatelet aggregation, antiviral and antiinflammatory actions. However, the anticancer effects of anthricin on A549 human nonsmall cell lung cancer cells and potential molecular mechanisms remain unknown. Therefore, we investigated the anticancer effect of anthricin and the underlying mechanism in A549 cells. Anthricin (10200 nM) inhibited the viability of A549 cells in a dose and timedependent manner. Moreover, anthricininduced apoptosis was confirmed by live and dead assay, 4,6dianmidino2phenylindole staining, and flow cytometric analysis. In addition, anthricin induced cell cycle arrest at the G2/M phase through suppression of the expression of cell cycle cascade proteins, Cdc2 and Cdc25C. Furthermore, it induced the expression of caspaserelated proteins and significantly suppressed the phosphorylation of insulinlike growth factor 1 receptor (IGF1R), PI3K and Akt. Anthricin significantly inhibited tumor growth without any significant change in the body weight of mice in A549 tumor xenograft BALB/c nude mice. Anthricin induced caspasedependent apoptosis through the IGF1R/PI3K/Akt signaling pathway in A549 cells.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Transducción de Señal
/
Carcinoma de Pulmón de Células no Pequeñas
/
Caspasas
/
Compuestos Heterocíclicos de 4 o más Anillos
/
Neoplasias Pulmonares
/
Antineoplásicos
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Oncol Rep
Asunto de la revista:
NEOPLASIAS
Año:
2018
Tipo del documento:
Article