TPC2 mediates autophagy progression and extracellular vesicle secretion in cancer cells.
Exp Cell Res
; 370(2): 478-489, 2018 09 15.
Article
en En
| MEDLINE
| ID: mdl-29990474
ABSTRACT
Autophagy is an evolutionarily conserved lysosomal degradation process, and is involved in various cellular processes. Here we studied the role of two pore channel 2 (TPC2), a lysosomal non-selective Na+/Ca2+ channel, in autophagy progression. We found that TPC overexpression in 4T1 mouse breast cancer cell line or in HeLa human cervical cancer cell line inhibited the fusion between autophagosome and lysosome, resulting in the accumulation of autophagosomes accompanied with increased lysosomal pH and TFEB nuclear localization. Interestingly, we also found that extracellular vesicle (EV) secretion was markedly decreased in TPC2 overexpressing cells but was induced in TPC2 knockdown cells. In addition, migration of TPC2 knockdown cells, not TPC2 overexpressing cells, was inhibited. Taken together, these results support a role of TPC2 in autophagy progression and EV trafficking in cancer cells.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Canales de Calcio
/
Núcleo Celular
/
Vesículas Extracelulares
/
Lisosomas
Límite:
Humans
Idioma:
En
Revista:
Exp Cell Res
Año:
2018
Tipo del documento:
Article
País de afiliación:
China