Albumin coated copper-cysteine nanozyme for reducing oxidative stress induced during sperm cryopreservation.
Bioorg Chem
; 80: 621-630, 2018 10.
Article
en En
| MEDLINE
| ID: mdl-30041138
ABSTRACT
In the present work, SOD mimetic nanozyme (NACu-Cys) consisting of Cu-Cys complex and nano-albumin (NA) were synthesized. After characterizing the nanozyme, its superoxide dismutase (SOD) behavior was evaluated by inhibition of the pyrogallol autoxidation method. The results revealed that NACu-Cys exhibited SOD mimetic activity with a half inhibition concentration (IC50) value of 7.0â¯×â¯10-3⯵M and a turnover number (kcat) of 5.4â¯×â¯107â¯s-1. In the next step, this nanozyme was applied as a protective agent against oxidative stress induced by sperm cryopreservation. Increasing the motility, raising the viability and reducing the apoptosis occurred as a result of NACu-Cys additions to human sperm freezing medium. Comparison between the natural SOD and SOD mimic behavior of NACu-Cys revealed that this nanoparticle has the ability to be used as oxidative stress decrescent during cryopreservation process.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Preservación de Semen
/
Espermatozoides
/
Albúmina Sérica Bovina
/
Cobre
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Cisteína
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Materiales Biomiméticos
/
Antioxidantes
Límite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Bioorg Chem
Año:
2018
Tipo del documento:
Article
País de afiliación:
Irán