Accurate quantification of CXCR4-using HIV-1 variants by Illumina deep-sequencing.
AIDS
; 32(16): 2429-2431, 2018 10 23.
Article
en En
| MEDLINE
| ID: mdl-30102658
ABSTRACT
Next-generation sequencing is a sensitive method for determining HIV-1 tropism but there is a lack of data on the quantification of X4 variants. We evaluated MiSeq and 454 GS-Junior platforms for determining HIV-1 tropism and for quantifying X4 variants. Both platforms were 93% concordant for determining HIV-1 tropism and correlated well for determining the proportion of X4 variants (Spearman correlation, ρâ=â0.748; Pâ<â0.0001). MiSeq Illumina sequencing seems to be well adapted for characterizing X4-containing samples.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Infecciones por VIH
/
Receptores del VIH
/
VIH-1
/
Receptores CXCR4
/
Tropismo Viral
/
Secuenciación de Nucleótidos de Alto Rendimiento
Tipo de estudio:
Evaluation_studies
Límite:
Humans
Idioma:
En
Revista:
AIDS
Asunto de la revista:
SINDROME DA IMUNODEFICIENCIA ADQUIRIDA (AIDS)
Año:
2018
Tipo del documento:
Article