Sortase A: A Model for Transpeptidation and Its Biological Applications.
Annu Rev Cell Dev Biol
; 34: 163-188, 2018 10 06.
Article
en En
| MEDLINE
| ID: mdl-30110557
ABSTRACT
Molecular biologists and chemists alike have long sought to modify proteins with substituents that cannot be installed by standard or even advanced genetic approaches. We here describe the use of transpeptidases to achieve these goals. Living systems encode a variety of transpeptidases and peptide ligases that allow for the enzyme-catalyzed formation of peptide bonds, and protein engineers have used directed evolution to enhance these enzymes for biological applications. We focus primarily on the transpeptidase sortase A, which has become popular over the past few years for its ability to perform a remarkably wide variety of protein modifications, both in vitro and in living cells.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Péptidos
/
Proteínas Bacterianas
/
Cisteína Endopeptidasas
/
Peptidil Transferasas
/
Aminoaciltransferasas
Límite:
Humans
Idioma:
En
Revista:
Annu Rev Cell Dev Biol
Asunto de la revista:
BIOLOGIA
Año:
2018
Tipo del documento:
Article