[Species Identification and Sequence Analysis of Plasmodium spp. in Border Areas of Yunnan Province by 18S rRNA-based Nested PCR]
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
; 34(3): 220-6, 2016 Jun.
Article
en Zh
| MEDLINE
| ID: mdl-30129721
ABSTRACT
Objective:
To analyze blood samples from patients with falciparum malaria and vivax malaria in border areas of Yunnan Province, using 18S rRNA-based nested PCR, and compare 18S rRNA sequences.Methods:
Blood or filter blood samples with positive microscopic results for Plasmodium falciparum or P. vivax infection were collected from Laza, Nankajiang, Mangdong and Nawei of Myanmar, and from Mengla, Tengchong and Yingjiang of Yunnan Province between 2004 and 2011. 18S rRNA-based nested PCR was conducted on the samples, and PCR products were sequenced and blasted. Phylogenetic tree was constructed using the neighbor-joining method with MEGA software (version 6.06).Results:
Microscopic examination revealed P. falciparum infection in 256 samples and P. vivax infection in 219 samples. The 18S rRNA-based PCR further confirmed P. falciparum infection in 242 samples, P. vivax infection in 176 samples, and mixed infection in 57 samples. The consistency rate was 81.7% ï¼388/475ï¼ between microscopic and PCR results. The inconsistency rate significantly correlated with parasite density ï¼Spearman's r=-0.408ï¼ P<0.05ï¼. Sequence alignment revealed 11 and 10 homologous sequences for P. falciparum and P. vivax 18S rRNA gene, comprising 2.9%ï¼6/205ï¼ and 22.5%ï¼27/120ï¼ variable sites, respectively. The 18S rRNA of P. falciparum clustered with that from Cameroonï¼GenBank accession number KC428742ï¼, but was distantly related with the S-type 18S rRNA from the Netherlands ï¼U36465ï¼ and Brazil ï¼U36466 and U36467ï¼. The 18S rRNA of P. vivax clustered with A-type 18S rRNA from Thailand ï¼U07367ï¼, but was distantly related with the C-type 18S rRNA from Thailandï¼U07368ï¼.Conclusion:
Nested PCR revealed mixed infection in 57 samples among those identified with single infection by microscopy. There is no significant difference in 18S rRNA sequence in seven counties/cities in Yunnan Province.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Filogenia
/
Plasmodium
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
Asunto de la revista:
PARASITOLOGIA
Año:
2016
Tipo del documento:
Article