[Toxoplasma gondii Rhoptry Protein 17 Inhibits the Apoptosis of Mouse Macrophages via Activation of Activator Protein 1 Signaling].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
; 34(6): 529-33, 2016 12.
Article
en Zh
| MEDLINE
| ID: mdl-30141842
ABSTRACT
Objective:
To investigate the effect of Toxoplasma gondii rhoptry protein 17ï¼ROP17ï¼ on γ-interferon (IFN-γ)-induced apoptosis of mouse J774A.1 monocyte macrophages.Methods:
The J774A.1 cells were transfected with recombinant plasmid p3×Flag-CMV-14/TgROP17 or empty plasmid p3×Flag-CMV-14. After addition of IFN-γ, flow cytometry and Western blotting were performed to detect apoptosis and the protein levels of phosphorylated c-Jun and apoptosis-related proteins cleaved Caspase-3, Bcl-2, Bcl-xL and Bcl-3. The p3×Flag-CMV-14/TgROP17 plasmid and c-Jun shRNA were co-transfected into J774A.1 cells, after which IFN-γ was added to induce cell apoptosis. The levels of cleaved Caspase-3 and Bcl-3 were analyzed using Western blotting.Results:
Flow cytometry showed that the apoptosis rate of cells overexpressing ROP17ï¼»ï¼3.73±0.51ï¼%ï¼½ was significantly lower than that of the control cellsï¼»ï¼7.78±1.10ï¼%, P<0.05ï¼½. Western blotting showed significant differences in protein levels of phosphorylated c-Junï¼0.196±0.028 vs. 0.075±0.010ï¼, Bcl-3ï¼0.461±0.063 vs. 0.108±0.013ï¼ and cleaved Caspase 3ï¼0.015±0.004 vs. 0.174±0.026ï¼ between the cells overexpressing ROP17 and control cells ï¼all P<0.05ï¼. However, the levels of Bcl-2 and Bcl-xL were not significantly different between the cells overexpressing ROP17 and the control. When the expression of c-Jun and phosphorylation of c-Jun were inhibited by c-Jun shRNA, the relative level of cleaved Caspase 3 in the RNA interferenced cells and control cells was 0.147±0.024 and 0.087±0.010, respectively (P<0.05), and the relative level of Bcl-3 was 0.085±0.010 and 0.162±0.011, respectively (P<0.05).Conclusion:
The anti-apoptosis effect of ROP17 is dependent on the phosphorylation of c-Jun and the expression of Bcl-3.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Toxoplasma
Límite:
Animals
Idioma:
Zh
Revista:
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
Asunto de la revista:
PARASITOLOGIA
Año:
2016
Tipo del documento:
Article